HPLC Analysis of Ribonucleic Acid Type VI from Torula Yeast on Comixshell Oligo Mixed-Mode Column

HPLC Analysis of Ribonucleic Acid Type VI from Torula Yeast on Comixshell Oligo Mixed-Mode Column

Column: Comixshell Oligo 2.7um 250*4.6mm (P/N: FMG-Oligo-EONU)

Mobile phase:  流動相A：ACN

流動相B：Water

流動相C：200mM 乙酸銨 pH5

Flow rate: 1 ml/min

Detection: 255 nm

案例說明：Comixshell Oligo可在UV和MS檢測器中分析RNA類特質(zhì)。相比一些離子交換柱要用到非揮發(fā)性鹽無法上質(zhì)譜，以及一些C18要用到高濃度TEAA并添加六氟異丙醇上質(zhì)譜，方便很多。