果汁中的維生素C在Comixsil HAX混合型柱上的HPLC定量分析

App ID: FLM-C210057

果汁中的維生素C在Comixsil HAX混合型柱上的HPLC定量分析

Equipment

Agilent 1100 HPLC system with DAD Analytical Balance

HPLC Column: Comixsil HAX 3 um 3.0x100 mm(P/N: FMD-HAX3-YONU)

Materials:

Fruit juices

ACN (Tedia, catalog #AS1127)

Distilled water (Aldrich, catalog #270733) Ammonium acetate (Aldrich, catalog #7262) Acetic acid (Aldrich, catalog #6283)

Vitamin C (Aldrich, catalog #A92902)

Method Description 2

HPLC Pump set up: A: DI water, B: Acetonitrile, C: 100 mM Ammonium acetate pH 4

Mobile phase composition: : A: 30%, B: 60%, C: 10%

Vitamin C Calibration and Sample Analysis

Column: Comixsil HAX 3 um  3.0x100 mm

Flow: 0.5 ml/min

Detection: 275 nm

Sample: Standards and juices

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